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This method has been successfully used by several groups studying Zika virus evolution and is facilitating an understanding of the spread of the virus in the Americas. Viral consensus sequences can be achieved starting with clinical samples in 1-2 days following a simple laboratory workflow. Our method relies on multiplex PCR for targeted enrichment of viral genomes from samples containing as few as 50 genome copies per reaction. The MinION protocol does not require an internet connection for analysis, making it suitable for field applications with limited connectivity. Here we present a protocol for generating coding-sequence complete genomes comprising an online primer design tool, a novel multiplex PCR enrichment protocol, optimised library preparation methods for the portable MinION sequencer (Oxford Nanopore Technologies) and the Illumina range of instruments, and a bioinformatics pipeline for generating consensus sequences. Select Reinstall OS X and click on the Continue button.Genome sequencing has become a powerful tool for studying emerging infectious diseases however, genome sequencing directly from clinical samples without isolation remains challenging for viruses such as Zika, where metagenomic sequencing methods may generate insufficient numbers of viral reads.Quit Disk Utility and return to the Utility Menu.Click on the Apply button, then wait for the Done button to activate and click on it.
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Set the Format type to Mac OS Extended (Journaled.).Click on the Erase icon in Disk Utility's toolbar.When Disk Utility loads select the drive (out-dented entry) from the Device list.Select Disk Utility from the Utility Menu and click on Continue button.Immediately after the chime hold down the Command and R keys until the Apple logo appears.
#Joshua atkinson apple mac repair install#
When the Utility Menu appears select Reinstall OS X then click on the Continue button.
Immediately after the chime hold down the Command and R keys until the Utility Menu appears.
Reset the System Management Controller (SMC).
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Disconnect all third-party peripherals and remove any support software like drivers and plug-ins.Shutdown the computer, wait 30 seconds, restart the computer.You should try each, one at a time, then test to see if the problem is fixed before going on to the next.īe sure to backup your files before proceeding if possible. A Troubleshooting Protocol to Identify Problems or Fix macOS El Capitan or Later